Charles Barnes, Ph.D

Director, Allergy/Immunology Laboratory

Telephone: (816) 931-8080
Fax: (816) 753-1307
Email: csbarnes@cctr.umkc.edu

Education/Experience
Ph.D.: University of Southern Mississippi. 1976

Post-Doctoral: Biochemistry Department. North Carolina State University

Affiliations
American Academy of Allergy, Asthma and Immunology
American Chemical Society
Pan-American Aerobiology Association

Interests
Dr. Barnes' clinical interests are in enzyme immunoassay and developing assays to evaluate exposure and sensitization to environmental allergens. Dr. Barnes' research interests are sequencing allergenic proteins from fungi.

Publications
Barnes C, Schreiber K, Pacheco F, Landuyt J , Hu F, Portnoy J. Measurement of allergens from outdoor air. Annals of Allergy Asthma and Immunology. 2000:104; 8-15

Portnoy J, Landuyt J, Pacheco F, Barnes C. A comparison of the Burkard and Allergenco MK-3 Volumetric Collectors. Annals of Allergy Asthma and Immunology 2000:104; 1-6.

Susan M. Flappan, Jay Portnoy, Patricia Jones, and Charles Barnes. Infant Pulmonary Hemorrhage in a Suburban Home with Water Damage and Stachybotrys. Environmental Health Perspectives Volume 107, Number 11, November 1999: P927-930

Garg U, Austin G, Barnes C, Hamilton M. Comparison of the Abbott IMx Tacrolimus I and Tacrolimus II Assays. Clin Chem. 1998, 44;1-2

Portnoy J, Brothers D, Pacheco F, Landuyt J, Barnes C. A double monoclonal antibody assay for the Alternaria Allergen, Alt a1. Annals of Allergy 1998, 81:59-64

Rosenthal D, Hu F, Pacheco F Landuyt J, Barnes C, Portnoy J. Northern blot. identification of mRNA containing sequence for protein allergen, Alt a1, in eight strains of Alternaria alternata. Annals of Allergy. 1998, 80:471-478

Goil S, Barnes C, Thibeault DW, Truog WE, A Simplified Method for Purification of Colostrum to Obtain Secretory Component Of IgA , Using Secretory Component as a Reference Protein in Tracheal Aspirate Fluid. Journal of Chromatography, Biomedical Applications. 1998, 705;203-211

Goil S, Truog WE, Barnes C, Thibeault DW. Isoprostanes in neonatal pulmonary diseases: Possible marker of lung injury. J Pediatrics. 1998, 132(2):349-351

Barnes C, F Pacheco, J Landuyt, D Rosenthal, Hu F, Portnoy. J. Production of a Recombinant protein from Alternaria containing the reported N- terminal of the Alt a1 allergen. in New Horizons in Allergy Immunotherapy. Proceedings of the International Symposium on the molecular biology of allergens and the atopic immune response 1996. Plenum Press New York:, Sehon A, Kraft D and HayGlass KT eds. Advances in Experimental Medicine and Biology Volume 409 : 197-203

Cage B, Schreiber K, Barnes C, Portnoy J. Evaluation of Four Bioaerosol Samplers in the Outdoor Environment. Ann Allergy. 1996, 77(1):1-7

Portnoy J, Bagstat K, Kanarek H, Pacheco F, Hall, B, Barnes CS. Premedication reduces the incidence of systemic reactions during inhalant rush immunotherapy with mixtures or allergenic extracts. Annals of Allergy 1994;73(5):409-418

Portnoy J, Pacheco F, Barnes C. Production of a double monoclonal antibody-based assay for the 70Kd Alternaria allergen, GP70. Ann Allergy. 1993; 71(4), 401-7.

Portnoy J, Pacheco F, Barnes C, Upadrashta B, Crenshaw R, Esch R. Selection of representative Alternaria strain groups based on morphology, enzyme profile, and allergen content. J Allergy Clin Immunol 1993;91:773-782.

Portnoy J, Pacheco F, Ballam Y, Barnes C. The effect of time and extraction buffers on residual protein and allergen content of extracts derived from four strains of Alternaria. J Allergy Clin Immunol 1993;91:130-38.

Barnes C, Upadrashta B, Pacheco F, Portnoy J. Enhanced sensitivity of immunoblotting with peroxidase conjugated antibodies using an immobilized substrate method. J Chromatography 1993;613(281-88)

Portnoy J, Pacheco F, Ballam Y, Barnes C. Separation of Alternaria into Protein and Carbohydrate Fractions Using Phenylsepharose. J Allergy Clin Immunol. 1991;87,789-93.

Portnoy J, Olson I, Pacheco F, Barnes C. Affinity Purification of a Major Alternaria Allergen Using a Monoclonal Antibody. Ann Allergy. 1990; 65,109- 14.